Detection of fimH virulence gene in uropathogenic Escherichia coli Using PCR

Urinary tract infections (UTIs) are one of main health problems caused by many microorganisms, including uropathogenic Escherichia coli (UPEC). The virulence factors important in colonization UPEC, extra-intestinal survival, and creation cytopathic effects. In addition, the expression special UPEC can contribute to uropathogenicity, as well worsening UTIs. this study, 134 isolates E. were collected from Baghdad/Iraq through March 2018 April 2019. identified culturing on Hardy chromo UTI agar confirmed at molecular level using uidA housekeeping gene real time polymerase chain reaction.The prevalence fimH was studied among 134uropathogenic patients attended Using reaction assay, Visualization (bp) 1.5% agarose gel analysis. By comparing bands a sample DNA ladder, their approximate sizes be determining. For instance, bright band above 1000 base pairs size for fimH. intended detected 94.8% absence 5.2%.of them.